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| Registros recuperados: 13 | |
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| Tu,Zhiming; He,Guangyuan; Li,Kexiu X.; Chen,Mingjie J.; Chang,Junli; Chen,Ling; Yao,Qing; Liu,Dongping P.; Ye,Huan; Shi,Jiantao; Wu,Xuqian . |
| This paper describes an efficient bacterial transformation system that was established for the preparation of competent cells, plasmid preparation, and for the storage in bacterial stocks in our laboratory. Using this method, a number of different plasmids have been amplified for further experiments. Competent cells for bacterial transformation were prepared by the calcium chloride method with an optimum concentration of 75 mM. Three different strains of Escherichia coli that were tested are DH5α, TG1 and XL1 blue, and the most efficient strain being XL1 blue. The optimal optical density (OD600) range for competent cell preparation varied for each of the strains investigated, and for XL1 blue it was 0.15-0.45; for TG1 it was 0.2-0.5; and for... |
| Tipo: Journal article |
Palavras-chave: Competent cells; E. coli; Plasmid; Storage; Transformation. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100014 |
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| Pedersen,Nina; Tuxen Poulsen,Thomas; Skovgaard Poulsen,Hans. |
| For promoter studies the cloning, subcloning and transfer to different plasmid vectors usually requires use of restriction enzymes and ligation reactions. One obstacle is the nucleotide polymorphisms of eukaryotic genomic DNA, which has the consequence that a sequence often differs from published sequences. Therefore sequencing, rigorous restriction enzyme analysis or introduction of suitable sites has to be performed prior to cloning and subcloning. In addition, conventional methods using restriction enzymes, insert purifications and ligations is expensive and labour demanding. We have developed a fast, efficient and inexpensive Cre recombinase-loxP based method, which allows cloning of promoter regions and subcloning of these into a variety of vectors in... |
| Tipo: Journal article |
Palavras-chave: Cloning; Cre recombinase; Plasmid; Reporter genes; Therapeutic genes. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200013 |
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| Mohamed,Mervat S. |
| The use of microorganisms in the degradation and detoxification of many toxic xenobiotics, especially pesticides, is an efficient tool for the decontamination of polluted sites in the environment. A novel bacterial strain (M1) was isolated from several water samples contaminated with methomyl which is capable of degrading methomyl pesticide (1000 ppm) in the presence of 0.05% glucose. These water samples were collected from different irrigation sites in Egypt where methomyl is heavily applied. The partial sequence of 16SrRNA gene of the isolate showed the highest similarity to Stenotrophomonas maltophilia. Restriction fragment patterns of isolated plasmid DNA showed that this strain harbours two different plasmids PMa (8Kb) and PMb (5Kb). PMb succeeded to... |
| Tipo: Journal article |
Palavras-chave: Carbamate; Methomyl; Plasmid; SPE-LC-ESI-MS; Stenotrophomonas. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400006 |
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| Schikorski, David; Renault, Tristan; Paillard, Christine; Bidault-toffin, A.; Tourbiez, Delphine; Saulnier, Denis. |
| The Gram-negative bacterium Vibrio harveyi is known to be highly pathogenic for the European abalone Haliotis tuberculata, which is a gastronomically important marine gastropod with a high commercial value. Since 1998, some particular bacterial strains are described as implicated in recurrent mortality outbreaks in French farm and field stocks of abalone. Recently, a 9.6kb plasmid named pVCR1, was shown to be harbored by one highly V. harveyi virulent ORM4 strain suggesting its involvement in virulence phenotype. Thus, we have developed in the present study two TaqMan real-time PCR assays allowing to (i) rapidly and specifically detect, by a duplex procedure and in less than 2 h, both V. harveyi and the presence of plasmid pVCR1 from unidentified bacterial... |
| Tipo: Text |
Palavras-chave: Vibrio harveyi; Haliotis tuberculata; Real-time PCR; Plasmid; Marine pathogen; Molecular diagnostic. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00124/23540/21383.pdf |
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| Donnelly,J.J.; Ulmer,J.B.. |
| DNA plasmids encoding foreign proteins may be used as immunogens by direct intramuscular injection alone, or with various adjuvants and excipients, or by delivery of DNA-coated gold particles to the epidermis through biolistic immunization. Antibody, helper T lymphocyte, and cytotoxic T lymphocyte (CTL) responses have been induced in laboratory and domesticated animals by these methods. In a number of animal models, immune responses induced by DNA vaccination have been shown to be protective against challenge with various infectious agents. Immunization by injection of plasmids encoding foreign proteins has been used successfully as a research tool. This review summarizes the types of DNA vaccine vectors in common use, the immune responses and protective... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plasmid; Nucleic acid; Protection; Antibodies; T cells; CTL. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200010 |
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| Pereira,Marcia de Oliveira; Rocha,Gabrielle de Souza; Lombardi,Simone dos Santos; Geller,Mauro; Pereira,Mário José; Santos-Filho,Sebastião David; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario. |
| The aim of this work was to evaluate the effect of fenoprofen on the labeling of blood constituents with technetium-99m, on the morphology of red blood cells and on the plasmid DNA. Blood samples from Wistar rats were incubated with fenoprofen and the assay of labeling of blood constituents with technetium-99m (99mTc) was performed. Blood cells, plasma, soluble and insoluble fractions of blood cells and plasma were separated. The radioactivity in each fraction was counted and percentage of incorporated radioactivity (%ATI) was determined. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of the red blood cells (RBC) was evaluated. Plasmid (pBSK) was incubated with fenoprofen with stannous chloride, and agarose gel... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Technetium-99m; Blood; Morphology; Plasmid; Fenoprofen. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700022 |
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| Nascimento,Elmiro R.; DaMassa,Al J.; Yamamoto,Richard; Nascimento,M. Graça F.. |
| One-hundred-five (105) clinical isolates of mycoplasma from caprine origin and one isolate from ovine were surveyed for plasmids, which were present in thirty-three (31%) of them. These mycoplasmas originated from 13 herds. Ten of them were symptomatic for mycoplasmal disease (mastitis, polyarthritis, septicemia) and three herds were asymptomatic, i.e., clinically normal. Twenty-eight isolates were Mycoplasma mycoides subspecies mycoides LC (large colony or caprine biotype), four were Mycoplasma capricolum subsp. capricolum and one was Mycoplasma cottewii. The isolated plasmids were linearized by EcoRI, EcoRV, EcoRI and EcoRV or BamHI and EcoRV, and were of five sizes (1.1, 1.6, 1.7, 1.8, and 1.9 Kbp). Based on restriction enzyme digestion and size of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasma; Plasmid; Goat; Endonuclease; Electrophoresis. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100006 |
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| Hernandez-Flores,Jose Luis; Pérez,Juan Caballero; Gutiérrez,Carlos Saldaña; Hernández,Andrés Cruz; Alonso,Gerardo Soto; Hernández,Sergio Pacheco; Gómez,Sergio Romero; Fernández,Francisco; Loske,Achim M.; Guillén,Juan Campos. |
| Abstract Multidrug-resistant microorganisms are of great concern to public health. Genetic mobile elements, such as plasmids, are among the most relevant mechanisms by which bacteria achieve this resistance. We obtained an Escherichia coli strain CM6, isolated from cattle presenting severe diarrheic symptoms in the State of Querétaro, Mexico. It was found to contain a 70 kb plasmid (pMEX01) with a high similarity to the pHK01-like plasmids that were previously identified and described in Hong Kong. Analysis of the pMEX01 sequence revealed the presence of a blaCTX-M-14 gene, which is responsible for conferring resistance to multiple β-lactam antibiotics. Several genes putatively involved in the conjugative transfer were also identified on the plasmid. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Plasmid; Antibiotic resistance; BlaCTX-M-14 gene. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300569 |
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| Registros recuperados: 13 | |
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